Regulated expression of proteins in mammalian cells stably transfected.

摘要

THE invention is in the field of genetic recombinants MATERIALS, ESPECIALLY FOR USE IN GENE THERAPY. VECTORS FOR TRANSFER USED IN ADDITION TO CELL GENETIC INFORMATION IN THE FIELD OF GENE THERAPY A VIRUS often rely on. A GROUP OF VIRUS HAS BEEN PROPOSED FOR USE FOR Transfection is the group of parvovirus, and in particular has proposed the use of adeno associated virus. The invention describes PROCEDURES AND ARRANGEMENTS FOR IMPROVED GENE THERAPY AND PREPARE FOR PRODUCTS FOR USE IN gene therapy BASED MATERIALS USING parvovirus. The invention describes a particular way regulated expression of genes under the control of the combination of a repressor and an activator RADICAL RADICAL IN PARTICULAR FOR EXPRESSION OF PRODUCTS ARE FOR TOXIC host cell WHICH ARE EXPRESSED IN. This makes it possible to achieve a stable transfection for protein expression TOXIC parvovirus, SO TO, DESCRIBED a cell line Encapsulation TO PRODUCE recombinant Parvovirus, INCLUDING adeno partners and VIRUSES RESULTING IN ITS INTERIOR.