System host / vector Escherichia coli in free antibiotic selection by complementation of an auxotroph.

摘要

A vector minimum prokaryotic expression which can not be homologously recombined with the genome of prokaryotic organisms containing e) an origin of replication, f) a marker auxotropia gene g) a promoter which is functionally active in prokaryotes and h) a foreign gene to that is expressed under the control of said promoter is particularly suitable for free selection of antibiotics.