PROCEDURE AND DEVICE FOR THE DETERMINATION OF PARAMETERS DEPENDING ON TEMPERATURE, SUCH AS THE PARAMETERS OF ASSOCIATION / DISPOSAL AND / OR THE CONSTANT OF BALANCE OF COMPLEXES CONSTITUTED BY AT LEAST TWO COMPONENTS.

摘要

Procedure for the parallel determination of temperature-dependent parameters, such as association / dissociation parameters and / or the equilibrium constant of complexes formed by at least two components, where the first components (12), which are in a liquid phase , are contacted with a large number of measuring points (10) in an optically excitable reaction medium and formed by second components (11) that are coupled to the solid reaction medium and specifically link with the first components (12), and this by contacting the liquid phase and the reaction medium (1) under conditions of complex synthesis (13) and the excitation of fluorescent dyes near the surface, linked to the first components (12) and / or second components (11), for the emission of fluorescent light (7) by irradiated excitation light (4) and detection of the fluorescent light emitted in a temperature spectrum variable flush, and the synthesis or disintegration of complexes, which include first components (12) and second components (11), is observed as a function of temperature.