DOUBLE-STRANDED HAIRPIN RNAS FOR RNAI

摘要

The invention is directed towards constructs for RNAi techniques. Theinvention provides a ribonucleic acid (RNA) for use as interfering RNA in genesilencing techniques to silence a target gene comprising in a 5' to 3'direction at least a first effector sequence, a second effector sequence, asequence substantially complementary to the second effector sequence and asequence substantially complementary to the first effector sequence, whereinthe complementary sequences are capable of forming double stranded regionswith their respective effector sequences and wherein at least one of thesesequences is substantially identical to the predicted transcript of a regionof the target gene, and a nucleic acid construct encoding such an RNA.