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D-1 LIKE DOPAMINE RECEPTOR ACTIVITY MODIFYING PROTEIN
D-1 LIKE DOPAMINE RECEPTOR ACTIVITY MODIFYING PROTEIN
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机译:D-1类多巴胺受体活性修饰蛋白
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摘要
A number of cDNA clones whose products may interact with D1 receptors in vivo were identified. One of the clones, P24, was characterized further. P24 is localized in dendtrites and spines of pyramidal cells in PFC. The extent of overlap between P24 expressing and D1 receptor expressing pyramidal cells appeared to be 100 %. In contrast, only a limited number D1 receptor antibody labeled neurons in caudate expressed P24. P24 lowers the threshold of D1 receptor response to dopamine (DA) by an order of magnitude. Sequence similarity suggests P24 is a diverged member of the RAMP family. The P24 protein is therefore referred to as a D1 DA RAMP, calcyon. The isolated protein and nucleotide molecule encoding the protein, as well as primers for the nucleotide, are described. The protein and compounds modifying DA binding to the receptor or calcium release which is mediated by the Calcyon, are useful in research studies, drug screening, and therapeutically.
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机译:鉴定了许多cDNA克隆,其产物可能在体内与D1受体相互作用。其中一个克隆,P24,被进一步表征。 P24位于PFC中锥体细胞的树突和棘中。表达P24和表达D1受体的锥体细胞之间的重叠程度似乎是100%。相反,仅有限数量的D1受体抗体标记的尾状神经元表达P24。 P24将D1受体对多巴胺(DA)的响应阈值降低一个数量级。序列相似性表明P24是RAMP家族的不同成员。因此,P24蛋白被称为D1 DA RAMP,钙体。描述了分离的蛋白质和编码该蛋白质的核苷酸分子,以及该核苷酸的引物。由Calcyon介导的修饰DA与受体结合或钙释放的蛋白质和化合物可用于研究,药物筛选和治疗。
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