METHODS OF DETECTING INTERACTIONS BETWEEN PROTEINS, PEPTIDES OR LIBRARIES THEREOF USING FUSION PROTEINS

摘要

The present invention discloses a method of reconstituting, folding, or reassembling peptides or other binding pairs into a functionally active protein or other complex using an antiparallel leucine zipper. The present invention also provides assays using fusion proteins comprising GFP fragments and test polypeptides for investigating protein-protein interactions.