NUCLEIC ACIDS AND METHODS FOR DETECTING VIRAL INFECTION, UNCOVERING ANTI-VIRAL DRUG CANDIDATES AND DETERMINING DRUG RESISTANCE OF VIRAL ISOLATES

摘要

Nucleic acid construct provides. The nucleic acid construct includes (one) expression cassette, comprising: (I) first polynucleotide region, including 5 " ' the ribonucleic acid virus of at least one end part core sequence of NCR sequence ribonucleic acid virus; (II) second polynucleotide region comprising 3 " ' UTR sequence ribonucleic acid virus and at least one C- end parts polymerase sequence virus; (III) third nucleotide area, encoding reporter molecule, third polynucleotide region, two sides are the first and second polynucleotide regions; (2) promoter sequence is operably coupled to expression cassette, in some sense, to make to transcribe strand RNA molecule from expression cassette.