METHOD FOR RECOVERING AND ANALYZING A CELLULAR COMPONENT OF CULTURED CELLS WITHOUT HAVING TO HARVEST THE CELLS FIRST

摘要

The present invention is summarized in that a cell extract suitable for recovery and analysis of a cellular component (a protein or a nucleic acid, for example) can be made by lysing the cells directly in the culture medium. The extract obtained can be used for subsequent applications such as protein recovery that were conventionally done with cell extracts of harvested cells. The elimination of the cell-harvest step makes these applications more amenable to high throughput adaptation.