MEDIUM FOR FEEDER-FREE DIFFERENTIATION AND FEEDER-FREE DIFFERENTIATION METHOD FROM PRIMATE EMBRYONIC STEM CELL

摘要

It is intended to provide a means whereby primate embryonic stem cells can be differentiated into blood cells or vascular endothelial cells substantially free from contamination with heterogenic animal cells, infection with a virus originating in a heterogenic animal, etc. Namely, a medium for feeder-free differentiation which contains a stroma cell adaptation medium; a culture system for feeder-free differentiation which comprises the above medium for feeder-free differentiation and a culture container coated with an extracellular matrix; a method of feeder-free differentiation from primate embryonic stem cells into blood cells, a method of feeder-free differentiation from primate embryonic stem cells into vascular endothelial cells, a method of producing blood cells, a method of producing vascular endothelial cells and a method of producing finger-like cells each using the above medium for feeder-free differentiation; and blood cells, vascular endothelial cells and finger-like cells obtained by these methods.