Systems and methods for measuring overlay error in a video-based augmented reality enhanced surgical navigation system are presented. In exemplary embodiments of the present invention the system and method include providing a test object, creating a virtual object which is a computer model of the test object, registering the test object, capturing images of control points on the test object at various positions within an augmented reality system's measurement space, and extracting positions of control points on the test object from the captured images, calculating the positions of the control points in virtual image, and calculating the positional difference of positions of corresponding control points between the respective video and virtual images of the test object. The method and system can further assess if the overlay accuracy meets an acceptable standard. In exemplary embodiments of the present invention a method and system are provided to identify the various sources of error in such systems and assess their effects on system accuracy. In exemplary embodiments of the present invention, after the accuracy of an AR system is determined, the AR system may be used as a tool to evaluate the accuracy of other processes in a given application, such as registration error.
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