PROBE FOR DETECTING Human herpesvirus, GENOTYPING KIT AND GENOTYPING METHOD FOR Human herpes virus USING THE SAME

摘要

PURPOSE: A probe for detecting human herpesvirus(HHV), a genotyping kit and a genotyping method for human herpesvirus using the same are provided, thereby rapidly, simply and accurately detecting HHV which consists of Herpes Simplex virus type 1(HSV-1), HSV-2 and human herpesvirus-8(HHV-8) and causes STD(sexual transmitted disease). CONSTITUTION: A probe for detecting human herpesvirus(HHV) is selected from oligonucleotides having the nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 9 and complementary oligonucleotides thereof and complementarily binds to the nucleic acid of human herpesvirus. A DNA chip for detecting human herpesvirus(HHV) or genotyping of HHV contains at least one probes selected from oligonucleotides having the nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 9 and complementary oligonucleotides thereof. A genotyping kit for human herpesvirus comprises (a) the DNA chip for detecting human herpesvirus, (b) a PCR primer and (c) a marker means for detecting amplified DNA hybridized with the DNA chip. A genotyping method for human herpesvirus comprises the steps of: (a) amplifying a sample DNA using the PCR primer; (b) hybridizing the amplified DNA on the DNA chip containing one probe or two or more of probe sets; and (c) detecting the product hybridized with the probe.