Microorganism for degrading trinitrotoluene and biological method for degrading trinitrotoluene using said microorganism

摘要

PURPOSE: A microorganism for degrading trinitrotoluene(TNT) and a biological method for degrading trinitrotoluene using the same microorganism are provided, which microorganism has improved TNT-degrading activity, so that wastewater, sewage or soil polluted by TNT can be biologically treated without resulting in environment pollution. CONSTITUTION: The microorganism Pseudomonas putida KPT202(KCCM 10473) having improved trinitrotoluene(TNT)-degrading activity is provided. The biological method for degrading trinitrotoluene comprises adding Pseudomonas putida KPT202(KCCM 10473) and at least one subsidiary energy source into the wastewater, sewage or soil polluted by TNT, and culturing it, wherein the subsidiary energy source is selected from subsidiary carbon source, subsidiary nitrogen source and Tween 80; the subsidiary carbon source is at least one selected from acetate, aspartate, citrate, corn steep liquor, glucose, glycerol, malic acid, molasses, pyruvate and succinate; and the subsidiary nitrogen source is ammonium chloride or potassium nitrate.