CULTURING OF PHELLINUS LINTEUS MYCELIUM BY INOCULATING PHELLINUS LINTEUS SPAWN INTO CULTURE MEDIUM CONTAINING CITRUS PULP AND BARLEY AND CULTURING

摘要

PURPOSE: A method of culturing Phellinus linteus mycelium by inoculating Phellinus linteus spawn into a culture medium containing citrus pulp and barley and then culturing is provided. It does not need to separate cultured mycelium from the culture medium and shortens extracting and purifying time. CONSTITUTION: Beer barley or barley is washed several times, soaked in water for 30min and steamed for 40min, followed by drying to a moisture content of about 50%. Then 100 parts by weight of steamed beer barley or barley is mixed with 3 to 60 parts by weight of citrus pulp, sterilized at 121deg.C for 1hr 20min and cooled to 20deg.C. Thereafter, 10ml Phellinus linteus spawn is inoculated into the barley medium and cultured at 25deg.C for 30 to 45 days.