PCR PRIMER SET FOR DETECTION OF HEPATITIS B TO REDUCE DETECTION TIME TO 30 MINUTES AND IMPROVE DETECTION REPRODUCTIVITY AND METHOD FOR DETECTING HEPATITIS B USING THE SAME

摘要

PURPOSE: A PCR primer set for detection of hepatitis B and a method for detecting hepatitis B using the same primer set are provided, thereby reducing the detection time to 30 minutes and improving the detection reproductivity by optimizing the PCR condition and using specific PCR primer set. CONSTITUTION: The PCR primer set for detection of hepatitis B is selected from the group consisting of: (a) a set of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2; (b) a set of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:3; (c) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:5; (d) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:6; (e) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:7; (f) a set of primers having the nucleotide sequences of SEQ ID NO:7 and SEQ ID NO:8; (g) a set of primers having the nucleotide sequences of SEQ ID NO:9 and SEQ ID NO:10; (h) a set of primers having the nucleotide sequences of SEQ ID NO:11 and SEQ ID NO:12; (i) a set of primers having the nucleotide sequences of SEQ ID NO:13 and SEQ ID NO:14; (j) a set of primers having the nucleotide sequences of SEQ ID NO:15 and SEQ ID NO:16; (k) a set of primers having the nucleotide sequences of SEQ ID NO:17 and SEQ ID NO:18; (l) a set of primers having the nucleotide sequences of SEQ ID NO:19 and SEQ ID NO:20; (m) a set of primers having the nucleotide sequences of SEQ ID NO:21 and SEQ ID NO:22; (n) a set of primers having the nucleotide sequences of SEQ ID NO:23 and SEQ ID NO:24; (o) a set of primers having the nucleotide sequences of SEQ ID NO:25 and SEQ ID NO:26; (p) a set of primers having the nucleotide sequences of SEQ ID NO:27 and SEQ ID NO:28; (q) a set of primers having the nucleotide sequences of SEQ ID NO:29 and SEQ ID NO:30; (r) a set of primers having the nucleotide sequences of SEQ ID NO:31 and SEQ ID NO:32; (s) a set of primers having the nucleotide sequences of SEQ ID NO:33 and SEQ ID NO:34; (t) a set of primers having the nucleotide sequences of SEQ ID NO:35 and SEQ ID NO:36; (u) a set of primers having the nucleotide sequences of SEQ ID NO:37 and SEQ ID NO:38; and (a) a set of primers having the nucleotide sequences of SEQ ID NO:39 and SEQ ID NO:40.