首页> 外国专利> IMMOBILIZED MICROORGANISM GIBBERELLA FUJIKUROI STRGA-NG39 PRODUCING GIBBERELLIN WITH HIGH YIELD FOR LONG PERIOD AND METHOD FOR PRODUCING GIBBERELLIN EMPLOYING THE SAME

IMMOBILIZED MICROORGANISM GIBBERELLA FUJIKUROI STRGA-NG39 PRODUCING GIBBERELLIN WITH HIGH YIELD FOR LONG PERIOD AND METHOD FOR PRODUCING GIBBERELLIN EMPLOYING THE SAME

机译:固定化微生物长时期生产高产赤霉素的赤霉素STRGA-NG39及其生产方法

摘要

PURPOSE: A microorganism Gibberella fujikuroi STRGA-NG39 and a method for producing Gibberellin employing the same microorganism are provided, thereby maintaining high yield of Gibberellin produced from immobilized Gibberella fujikuroi STRGA-NG39 for a long period. CONSTITUTION: The microorganism Gibberella fujikuroi STRGA-NG39(KCTC 10196BP) is provided, wherein Gibberella fujikuroi STRGA-NG39(KCTC 10196BP) is produced by inducing mutation of a Gibberellin producing microorganism isolated from the rice plants infected by Bakanae disease with NTG as a mutation inducing material. The method for producing Gibberellin comprises the steps of: (i) immobilizing Gibberella fujikuroi STRGA-NG39(KCTC 10196BP) into a carrier; (ii) culturing the immobilized Gibberella fujikuroi STRGA-NG39(KCTC 10196BP) in a medium, centrifuging the cultured medium, collecting the supernatant, and adding the same amount of fresh medium as the supernatant into the microorganism; (iii) repeating the step (ii) 5 to 10 times and collecting the cultured medium; and (iv) obtaining Gibberellin from the cultured medium.
机译:用途:提供了一种微生物的富士赤霉菌STRGA-NG39和一种利用相同微生物生产赤霉素的方法,从而长期保持了由固定化的富士赤霉菌STRGA-NG39生产的赤霉素。组成:提供了一种微生物的富士赤霉菌STRGA-NG39(KCTC 10196BP),其中通过诱导从产自巴卡那病感染的水稻植株中分离出的产生赤霉素的微生物的突变,以NTG作为突变体产生了富士赤霉菌STRGA-NG39(KCTC 10196BP)。诱导物质。生产赤霉素的方法包括以下步骤:(i)将富士赤霉菌STRGA-NG39(KCTC 10196BP)固定在载体中; (ii)在培养基中培养固定化的藤本赤霉菌STRGA-NG39(KCTC 10196BP),离心培养基,收集上清液,并向微生物中加入与上清液相同量的新鲜培养基; (iii)重复步骤(ii)5至10次并收集培养基; (iv)从培养基中获得赤霉素。

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