首页> 外国专利> alpha;-GLUCAN PHOSPHOLYASE ORIGINATED THERMUS CALDOPHILUS GK24, PREPARATIVE METHOD THEREOF USING RECOMBINANT HOST, AND SYNTHETIC METHOD OF alpha;-D-GLUCOSE-1-PHOSPHATE USING THE SAME

alpha;-GLUCAN PHOSPHOLYASE ORIGINATED THERMUS CALDOPHILUS GK24, PREPARATIVE METHOD THEREOF USING RECOMBINANT HOST, AND SYNTHETIC METHOD OF alpha;-D-GLUCOSE-1-PHOSPHATE USING THE SAME

机译：α;-葡聚糖磷酸酶原产的嗜热杯状线虫GK24，使用重组宿主的制备方法，以及使用相同方法合成α; -D-葡萄糖-1-磷酸的方法

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摘要

The present invention is an alpha-glucan phosphorylase (α-glucan phospholyase) relates to, specifically, the thermophilic strain of more commerce knife pillar's (Thermus caldophilus) alpha described in SEQ ID NO: 1 isolated from GK24 strain-glucan phosphorylase, a gene encoding the enzyme, a vector containing the gene, transformed with the recombinant strain with the vector, alpha with a recombinant strain method for producing a glucan phosphorylase and the alpha-glucan phosphorylase using the alpha-D-relates to method for the synthesis of glucose-1-phosphate (α-D-glucose-1-phosphate). Since glucan phosphorylase is heat resistant, the use of the enzyme alpha from cheap starch-alpha of the present invention de-bacteria only can significantly improve the production yield of the glucose-1-phosphate as in the pre-production process of the product it is possible to prevent contamination from.

机译：本发明是一种α-葡聚糖磷酸化酶（α-葡聚糖磷酸化酶），特别地，涉及从GK24分离的SEQ ID NO：1中描述的更多商业刀柱的（Thermus caldophilus）α的嗜热菌株。菌株-葡聚糖磷酸化酶，编码该酶的基因，包含该基因的载体，用带有该载体的重组菌株转化，用重组菌株方法生产α-葡聚糖磷酸化酶和使用α-D-relates的α-葡聚糖磷酸化酶合成葡萄糖-1-磷酸（α-D-葡萄糖-1-磷酸）的方法。由于葡聚糖磷酸化酶是耐热的，因此与本产品的预生产过程一样，使用廉价的本发明淀粉-α-细菌的酶α-酶只能显着提高葡萄糖-1-磷酸的生产产率。可以防止污染。 展开▼

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公开/公告号KR20050051055A

专利类型

公开/公告日2005-06-01

原文格式PDF

申请/专利权人 KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY;
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申请/专利号KR20030084740

发明设计人 KIM JOONG SU;PARK JUNG EUN;PARK BO HYUN;LEE DAE SIL;SHIN HYUN JAE;KIM DOO IL;KOH SUK HOON;BAE JUNG DON;
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申请日2003-11-26

分类号C12N9/12;

国家 KR

入库时间 2022-08-21 22:05:14

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1. 来自 C a n d i d a A l bi c a n s的甘油－3－磷酸磷酸酶和甘油－3－磷酸脱氢酶 ,编码它们的基因, 包含该基因的载体和宿主细胞, 和使用该宿主细胞生产甘油的方法 [P] . 中国专利： CN1798835A . 2006-07-05

2. 生物样品中(1→3)-β-D-葡聚糖的免疫分析方法、(1→3)-β-D-葡聚糖分析试剂盒以及(1→3)-β-D-葡聚糖免疫分析方法中使用的生物样品碱性预处理溶液 [P] . 中国专利： CN112424602A . 2021-02-26

3. HEAT-RESISTANT ALPHA-GLUCOSIDASE OF THERMOPHILE, THERMUS CALDOPHILUS GK24 AND METHOD FOR MANUFACTURING THE SAME [P] . 韩国专利： KR20010035734A . 2001-05-07

机译：耐热性α-葡糖苷酶，嗜热线虫GK24及其制造方法

4. METHOD FOR PRODUCING A RECOMBINANT ALPHA-GALACTOSIDASE OF THERMUS SP. T2 IN HOST CELLS [P] . ES2172380A1 . 2002-09-16

机译：产生Thermus SP的重组α-半乳糖苷酶的方法。 T2在主机中

5. METHOD FOR PRODUCING A RECOMBINANT ALPHA-GALACTOSIDASE OF THERMUS SP. T2 IN HOST CELLS [P] . 世界知识产权组织专利： WO0164914A1 . 2001-09-07

机译：产生Thermus SP的重组α-半乳糖苷酶的方法。 T2在主机中

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