METHOD FOR REFOLDING PROTEINS USING SIMULATED MOVING BED CHROMATOGRAPHY

摘要

The present invention includes the steps of preparing, and more particularly: 1) a target protein sample relates to a method of refolding the protein aggregates (inclusion body) using SMB chromatography; 2) a step of loading (loading) the protein samples of filling a filler in a column of SMB chromatography and step, and refolding (refolding); And 3) it relates to a method of refolding protein aggregates by injecting a refolding buffer to the column includes the step of separating the refolded protein from step 2. A method of refolding the protein aggregates of the present invention can be obtained by the continuous refolding the protein with only partial separation, can greatly contribute to cost reduction in the production of high yields it can be expected useful enzyme.