MANUAL METHOD OF LIQUID-BASED CERVICAL CYTOLOGY

摘要

The present invention is a liquid cell, while minimizing the defect rate resulting from the slide to overcome the important elements of nuclear chromatin lack of observation of the microscopic structure of the read to improve the quality of the test results, cervical liquid cell handwritten one to contribute to the test cost reduction test It relates to a method,; The using preparative purchased specimen material itself brush collected the sample from the cervix, and the sample is collected brush comprising: receiving a preservative and; The step of shaking the sample contained the preservative container or tap on a table floating cells in the preservative and; Using a multi-pipette to dispense 20% sugar 4㎖ 15㎖ the centrifuge tube, and the step using the serum separation tube for dispensing the sample 8㎖ on the upper side of 20% sugar and; The method comprising centrifuged for 2 min at 1000rpm in a busy state; Step ll by inhalation using the inhaler of the supernatant and 8㎖; The remaining 4㎖ separated 10 minutes and centrifuged at 2000rpm to form a needle things on the floor and a centrifuge tube; Step discard the supernatant and all; Centrifuge tube to form a cell suspension was dispensed to the buffer 6㎖ over needle objects formed in the bottom, and then, after allowed to stand for 10 minutes to form a needle things on the floor and separated again 10 minutes and centrifuged to 2000rpm; The step of dropping the object by the needle after repeated pipette operations dispense the buffer 1000㎕ a centrifuge tube using a pipette from the bottom centrifuge tube; The method comprising using a pipette, the cell suspension was added dropwise over 400㎕ attached to the circular specimen dispensing framework of 1.5㎝ slide diameter, and dropping the additional buffer and 600㎕; After allowed to stand for 15 minutes with a dropping state buffer solution twice, and then washed 3 times in alcohol comprising: slicer bonded fixed in 95% ethyl alcohol and; It is characterized by comprising the step of using the staining reagents to be used for the liquid cell slide to dye the conventional pop smear slide microscopy after pop stain.