Method for detecting endocrine disruptors using yeast two-hybrid system containing hER? gene

摘要

The present invention is the ability to activate transcription (transcription) of the dependent target gene (target gene) to, more specifically to the ligand (ligand) relates to a method for detecting a chemical substance having a human endocrine disorders activity using the yeast nucleus as with the characteristics of the receptor according to the method of detecting a chemical (estrogenic chemicals) having an endocrine disorder activity of the estrogen receptor β and the transfer-conjugated factor (co-activator) a human-derived yeast dual-hybrid system (two-hybrid system) and introducing the expression on, by monitoring in which expression of the reporter gene is introduced into a chromosome of a method for detecting estrogen-like substances having an endocrine disorder activity by measuring the ligand-dependent transcriptional activation of estrogen receptors will be. According to the method of the present invention can be detected as a mammal or fish such as simple a more specific chemicals for a number of chemicals that affect the endocrine system of humans or animals in the environment without having to use and rapid biological methods .