Method for discriminating endocrine disruptors using transgenic yeast by two-hybrid system containing hER ?gene or co-activator SRC1 gene

摘要

The present invention relates to a determining method of the endocrine disruptor using the yeast transformant of the present invention is one that includes the estrogen receptor α gene of human origin - with a hybrid system and a transfer-conjugated factor SRC1 transgenic transgenic yeast relates to a method for determining endocrine disruptors using a hybrid system by expressing the reporter genes in yeast double that of endocrine disorders of various active chemicals can be measured and determined. Accordingly, the present invention the double-hybrid system is a mammalian cell and to overcome the difference in yeast between the transfer tool mammals or fish, such as a not environmental human or animal for a number of chemicals that exist in without using the introduction of transfer-conjugated factor there is a certain endocrine chemicals affect the excellent effects that can be more convenient and faster to determine the biological method.