A method of high cell-density culture of Escherichia coli by overproducing Mlc protein

摘要

The invention relates to a method for over-expressing a regulator of the carbohydrate metabolism Mlc protein of Escherichia coli. More specifically, the present invention provides a Mlc overexpressing mutant strain produced by the method of over-expressing the Mlc protein by inducing a mutation to the transcription control region of the mlc gene in the E. coli -10 region and SD sequence a ribosome binding site, the method comprising. Can be by lowering the metabolic rate and specific growth rate per E. coli in the glucose added culture medium due doemeuro Mlc protein over-expression by suppressing the production of acetate toxic metabolic by-product of glucose metabolism in the culture of E. coli at a high concentration according to the invention, Mlc overexpressing mutant strain produced by the present invention can increase the production of recombinant proteins are used as the foreign gene, expression host cells.