Method of culturing somatic embryo of broccoli and method for the mass-production of broccoli sprouts using the same

摘要

PURPOSE: A method for culturing a somatic embryo of broccoli and a method for mass-producing broccoli sprouts using the same are provided, thereby from the somatic embryo of broccoli rapidly and easily mass-producing broccoli sprouts which are used in anticancer foods or medicines. CONSTITUTION: A method for culturing a somatic embryo of broccoli comprises the steps of: (1) culturing broccoli tissue which is selected from seeds, anther, root, stem and leaf of broccoli and is capable of forming an embryo in a medium to produce the broccoli embryo; (2) cutting the broccoli embryo and culturing it on 2,4-D containing medium to form callus of broccoli; (3) forming embryo-developed cells from the callus; and (4) subculturing the embryo-developed cells in a hormone free medium. A method for mass-producing broccoli sprouts comprises the steps of: (1) selecting embryo-developed cells and culturing them; (2) proliferating the embryo-developed cells and isolating cell mass; and (3) mass-culturing the cell mass.