A nonradioisotopic assay method to measure Cdc2 kinase activity

摘要

PURPOSE: A method for nonradioisotopic assay of Cdc2 kinase activity is provided, thereby resolving problems caused by using radioisotope in the Cdc2 kinase activity assay, so that the Cdc2 kinase activity can be stably and easily assayed. CONSTITUTION: A method for nonradioisotopic assay of Cdc2 kinase activity comprises the steps of: mixing Cdc2 kinase with retinoblastoma protein Rb to phosphorylate the retinoblastoma protein; reacting the retinoblastoma protein with an antibody capable of recognizing phosphorylated serine at amino acid position 795 and around amino acid residues in human retinoblastoma protein Rb; and subjecting the reaction result to Western blotting, wherein the antibody is 795-phospho-Ser detecting antibody.