METHOD genetic typing of strains of Mycobacterium tuberculosis complex

摘要

1.a genetic u0442u0438u043fu0438u0440u043eu0432u0430u043du0438u00a0 u043cu0438u043au043eu0431u0430u043au0442u0435u0440u0438u0439 tuberculosis complex strains, including the allocation of genome dna, u0430u043cu043fu043bu0438u0444u0438u043au0430u0446u0438u044e u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu0445 loci dna with u0444u043bu0430u043d u043au0438u0440u0443u044eu0449u0438u043cu0438 their u043fu0440u0430u0439u043cu0435u0440u0430u043cu0438 in selected reaction u0441u043cu0435u0441u00a0u0445 with subsequent u0434u0435u0442u0435u043au0446u0438u0435u0439 pcr products and the numbers u0430u043bu043bu0435u043bu00a0 in accordance with the formula " the size of pcr - product, the number and size of tandem duplications u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 orderwhat a strong u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu0445 loci dna using u043bu043eu043au0443u0441u044b v1, v2, v3 and v4, and u0444u043bu0430u043du043au0438u0440u0443u044eu0449u0438u0435 their u043fu0440u00a0u043cu044bu0435 (u) and reverse (r) u043fu0440u0430u0439u043cu0435u0440u044b have the following nucleotide p u043eu0441u043bu0435u0434u043eu0432u0430u0442u0435u043bu044cu043du043eu0441u0442u0438:;V1u GAATTCTTCGGTGGTCTCGAGTG,;V1r CTACGGTGTAGCGTCGCTGAC;;V2u TACACGCAGCTGGAAAGTCCAG;V2r GGTCGTTGGTCTAGCCAGTGG;V3u ACGGAAGGAATACTCAGCGGAG;V3r CTTCAGTCTGCCGGCAATAACG;V4u CGGTATCCTGATGTTAATCGTAAGG;V4r CCATCCCACTGAGCGTCGAAG;2. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 1, so that the u0434u043bu00a0 each test strain u0441u043eu0441u0442u0430u0432u043bu00a0u044eu0442 four reaction mixtures containing a few u043fu0440u0430u0439u043cu0435u0440u043eu0432, u0444u043bu0430u043du043au0438u0440u0443u044eu0449 it is one of the four analysed loci.;3. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 1, so that the u043eu043fu0440u0435u0434u0435u043bu00a0u0435u043cu044bu043c u0430u043bu043bu0435u043bu00a0u043c) four digit number, u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u044eu0449u0438u0439 u0430u043bu043bu0435u043bu044cu043du044bu0439 profile in accordance with the number of tandem on u0432u0442u043eu0440u043eu0432 loci in v1, v2, v3, v4.;4. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 1, so that the number u0430u043bu043bu0435u043bu00a0 u0434u043bu00a0 each of the four loci u043eu043fu0440u0435u0434u0435u043bu00a0u044eu0442 formula: v1 (n 51, 53; v2: (n = 93): 111; v3: (n = 63): 78; v4: (n = 100): 69, where n is the size of the fragment dna, para. oh.