METHOD FOR PRODUCING VACCINES AGAINST ASSOCIATED salmonellosis, colibacillosis nutria

摘要

method of u0438u0437u0433u043eu0442u043eu0432u043bu0435u043du0438u00a0 vaccine against u0441u0430u043bu044cu043cu043eu043du0435u043bu043bu0435u0437u0430 and u043au043eu043bu0438u0431u0430u043au0442u0435u0440u0438u043eu0437u0430 u043du0443u0442u0440u0438u0439, comprising a u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du043eu0439 can u0438u043du0430u043au0442u0438u0432u0430u0446u0438u044e u0444u043eu0440u043cu0430u043bu0438u043du043e m, the u0430u0434u044au044eu0432u0430u043du0442u0430 u0433u0438u0434u0440u043eu043eu043au0438u0441u0438 u0430u043bu044eu043cu0438u043du0438u00a0, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, separately grown strain Salmonella typhimurium containing antigen 04 and strain of escherichia coli containing u0430u043du0442u0438u0433 yong 01the local u044du043fu0438u0437u043eu043eu0442u0438u0447u0435u0441u043au043eu0433u043e hearth in liquid nutrient environment with the addition of glucose and 0.2% to the concentration of microbial cells in 1 ml of 4 - 5 billion, as of alina to 0.4 - 0.6%, and the concentration and temperature 37u0441 within 48 to 72 h, mixing strains in equal u0441u043eu043eu0442u043du043eu0448u0435u043du0438u00a0u0445, appendix u0433u0438u0434u0440u043eu043eu043au0438u0441u0438 u0430u043bu044eu043cu0438u043du0438u00a0 in the amount of 15% to the volume of the culture, and thoroughly mix.vialing up and u0443u043au0443u043fu043eu0440u0438u0432u0430u044eu0442.