METHOD FOR PRODUCING brucellosis DIAGNOSTICUM

摘要

1. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 u0431u0440u0443u0446u0435u043bu043bu0435u0437u043du043eu0433u043e u0434u0438u0430u0433u043du043eu0441u0442u0438u043au0443u043cu0430 comprising flush microbial mass of brucella abortus 19 wa. u043du0430u0442u0440u0438u00a0 chloride, u0438u043du0430u043au0442u0438u0432u0430u0446u0438u044e microbial mass, its u043au043eu043du0441u0435u0440u0432u0430u0446u0438 yu and staining, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, flush microbial mass u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 0.9% of u043du0430u0442u0440u0438u00a0 chloride solution, with u0434u043eu0432u043eu0434u00a0u0442 its concentration up to 20 - 30 billion m./ ml, u0438u043du0430u043au0442u0438u0432u0430u0446u0438u044e microbial suspensions u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 1% formalin solution at a temperature of 22u0441 within 2 h, the staining u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 0.04% of saline haematoxylin and u043au0430u0440u0430u0446u0438, which u0434u043eu0431u0430u0432u043bu00a0u044eu0442 equally to its draught and're warming their temperature 56u0441 for 16 to 18 hours after u043eu043au0440u0430u0448u0438u0432u0430u043du0438u00a0 and u0446u0435u043du0442u0440u0438u0444u0443u0433u0438u0440u0443u044eu0442 in 2000 on the / mines for 15 min.then the supernatant u0446u0435u043du0442u0440u0438u0444u0443u0433u0438u0440u0443u044eu0442 at 7000 rpm for 30 min, then the residue u0440u0435u0441u0443u0441u043fu0435u043du0434u0438u0440u0443u044eu0442 in 0.9%, and u0446u0435u043du0442u0440u0438u0444u0443u0433u0438u0440u0443u044eu0442 u043du0430u0442u0440u0438u00a0 chloride solution in the same u0443u0441u043bu043eu0432u0438u00a0u0445 3 four times followed by the draught u0434u043eu0431u0430u0432u043bu00a0u044eu0442 0.1% solution of formalin to the original volume and the stabilizer of 0.1 ml to 1 ml of the u0434u0438u0430u0433u043du043eu0441u0442u0438u043au0443u043cu0430 after hours it u0434u0438u0430u0433u043du043eu0441u0442u0438u043au0443u043c u043bu0438u043eu0444u0438u043bu044cu043du043e dried.;2. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 1, so that, as the stabilizer using 1.5% solution u043fu043eu043bu0438u0432u0438u043du0438u043bu043fu0438u0440u0440u043eu043bu0438u0434u043eu043du0430 and 7% solution u0441u0430u0445u0430u0440u043eu0437u044b, u0432u0437u00a0u0442u044bu0435 in the ratio of 1: 1.;3. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 1, so that, as the stabilizer using 0.03% u043au0430u0440u0431u043eu043du0430u0442u043du044bu0439 buffer and 3% solution u0441u0430u0445u0430u0440u043eu0437u044b, u0432u0437u00a0u0442u044bu0435 in the ratio of 1: 1.