A method for detecting Salmonella antigen in smears BY immunofluorescence

摘要

method u043eu0431u043du0430u0440u0443u0436u0435u043du0438u00a0 u0441u0430u043bu044cu043cu043eu043du0435u043bu043bu0435u0437u043du043eu0433u043e antigen in smears - fingerprints method of reaction u0438u043cu043cu0443u043du043eu0444u043bu044eu043eu0440u0435u0441u0446u0435u043du0446u0438u0438, including preparation of diagnostic medicine u0434u043bu00a0 u043fu0440u043eu0432u0435u0434u0435u043du0438u00a0 reaction u0438u043cu043cu0443u043du043eu0444u043bu044eu043eu0440u0435u0441u0446u0435u043du0446u0438u0438, u0434u043bu00a0 which set u043au0440u0430u0441u00a0u0449u0438u0439 titer diagnostic drug, the scarred u0444u043bu044eu043eu0440u043eu0445u0440u043eu043cu043eu043c, u0440u0430u0437u0432u043eu0434u00a0u0442, drain and u043eu0441u0443u0449u0435u0441 u0442u0432u043bu00a0u044eu0442 direct production reaction u0438u043cu043cu0443u043du043eu0444u043bu044eu043eu0440u0435u0441u0446u0435u043du0446u0438u0438, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 orderwhat u0434u043bu00a0 u043eu0431u043du0430u0440u0443u0436u0435u043du0438u00a0 u0441u0430u043bu044cu043cu043eu043du0435u043bu043bu0435u0437u043du043eu0433u043e antigen in smears - fingerprints using u043fu0440u00a0u043cu0443u044e reaction u0438u043cu043cu0443u043du043eu0444u043bu044eu043eu0440u0435u0441u0446u0435u043du0446u0438u0438, a diagnostic drug u0434u043bu00a0 pic u0442u0430u043du043eu0432u043au0438 u043fu0440u00a0u043cu043eu0439 reaction u0438u043cu043cu0443u043du043eu0444u043bu044eu043eu0440u0435u0441u0446u0435u043du0446u0438u0438 use u043bu0435u043eu0444u0438u043bu0438u0437u0438u0440u043eu0432u0430u043du043du044bu0439 diagnostic fluorescent u0441u0430u043bu044cu043cu043eu043du0435u043bu043bu0435u0437u043du044bu0439 immunoglobulin to antigens a, b, d, sword u0435u043du044bu0439 u0444u043bu044eu043eu0440u043eu0445u0440u043eu043cu043eu043c,in the setting of u043au0440u0430u0441u00a0u0449u0435u0433u043e u0442u0438u0442u0440u0430 through u043fu0440u00a0u043cu043eu0439 reaction u0438u043cu043cu0443u043du043eu0444u043bu044eu043eu0440u0435u0441u0446u0435u043du0446u0438u0438, depending on which a fluorescent diagnostic drug, poorly u0444u043bu044eu043eu0440u043eu0445u0440u043eu043cu043eu043c, used against u0433u043bu043eu0431u0443u043bu0438u043du043eu0432 various biological objects, select the appropriate response will focus on the series) u0434u043bu00a0 u043fu0440u00a0u043cu043eu0439 u0438u043cu043cu0443 u043du043eu0444u043bu044eu043eu0440u0435u0441u0446u0435u043du0446u0438u0438 antigen u0440u0430u0437u0432u043eu0434u00a0u0442 1: 1000then a fluorescent diagnostic medicine in multiples of u0440u0430u0437u0432u0435u0434u0435u043du0438u00a0u0445 from 1: 2 to 1: 1024 u043du0430u043du043eu0441u00a0u0442 on the prepared antigen, after the u043eu0442u043cu044bu0432u0430u043du0438u00a0 from u043du0435u0441u0432u00a0u0437u0430u043du043du044bu0445 antibodies couple re times u043fu00a0u0442u044c minutes with saline preparations subjected to microscopy on phosphorescent microscope set two related u0440u0430u0437u0432u0435u0434u0435u043du0438u00a0 u0444u043bu044eu043eu0440u0435u0441u0446u0438u0440 u0443u044eu0449u0435u0433u043e diagnostic drugin which the difference in the effect of the drug, and u00a0u0432u043bu00a0u0435u0442u0441u00a0 u043au0440u0430u0441u00a0u0449u0438u043c u0442u0438u0442u0440u043eu043c u0444u043bu044eu043eu0440u0435u0441u0446u0438u0440u0443u044eu0449u0435u0433u043e diagnostic drug, with u043au0440u0430u0441u00a0u0449u0438 s titer, count how many times i get to he had a titer of 1: 32, which is mostly shown in the u0430u043cu043fu0443u043bu0435 with u043bu0435u043eu0444u0438u043bu0438u0437u0438u0440u043eu0432u0430u043du043du044bu043c diagnostic u0444u043bu044eu043eu0440u0435u0441u0446u0438u0440u0443u044e with u0441u0430u043bu044cu043cu043eu043du0435u043bu043bu0435u0437u043du044bu043c substances to antigens a, b, du043cu0435u0447u0435u043du044bu043c u0444u043bu044eu043eu0440u043eu0445u0440u043eu043cu043eu043c, breeding against u0441u0430u043bu044cu043cu043eu043du0435u043bu043b u043fu0440u043eu0438u0437u0432u043eu0434u00a0u0442 so that he had the breeding 1: 16, an amp with u043bu0435u043eu0444u0438u043bu0438u0437u0438u0440u043eu0432u0430u043du043du044bu043c u0434u0438u0430u0433u043du043eu0441u0442u0438u0447u0435u0441u043a they u0441u0430u043bu044cu043cu043eu043du0435u043bu043bu0435u0437u043du044bu043c fluorescent substances to antigens a, b, d, u043cu0435u0447u0435u043du044bu043c u0444u043bu044eu043eu0440u043eu0445u0440u043eu043cu043eu043c, u0432u043du043eu0441u00a0u0442 500 mkl u043fu043eu0434u0449u0435u043bu043eu0447u0435u043du043du043eu0439 distilled waterthen in the centrifuge tube to mix the required amount of u043bu0435u043eu0444u0438u043bu0438u0437u0438u0440u043eu0432u0430u043du043du043eu0433u043e diagnostic u0444u043bu044eu043eu0440u0435u0441u0446u0438u0440u0443u044eu0449u0435u0433u043e u0441u0430u043bu044cu043cu043eu043du0435u043bu043bu0435u0437u043du043eu0433u043e immunoglobulin to u0430u043du0442u0438u0433 u0435u043du0430u043c a, b, d, the scarred u0444u043bu044eu043eu0440u043eu0445u0440u043eu043cu043eu043c, u0430u0446u0435u0442u043eu043du043eu0432u043eu0433u043e powder and distilled water or u0434u0438u0441u0442u0438u043bu043bu0438u0440u043eu0432u0430u043du043du043eu0433u043e solution with ph 7.2.7.4, then divorced diagnostic medicine are the depletion of u0430u0446u0435u0442u043eu043du043eu0432u044bu043c u043fu0435u0447u0435u043du043eu0447u043du044bu043c powder in 1 ml of diagnostic dose 80 mg powder, u043fu0440u0438u0433u043eu0442u043eu0432u043bu0435 u043du043du044bu043c from liver of swine during u0438u0441u0442u043eu0449u0435u043du0438u00a0 u043au0440u0430u0441u00a0u0449u0438u0439 titer diagnostic drugs reduce two times and get the right work breeding 1: 16in the absence of opportunities to acquire u0441u0430u043bu044cu043cu043eu043du0435u043bu043bu0435u0437u043du044bu0439 antigen then cook a few u0440u0430u0437u0432u0435u0434u0435u043du0438u0439 diagnostic drug on physiological solution ph 7.2...7.4 - 1 + 31, 1 + 15, + 7, + 3, 1, 1, 1 + 1 and u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 depletion of each u0440u0430u0437u0432u0435u0434u0435u043du0438u00a0 u0430u0446u0435u0442u043eu043du043eu0432u044bu043c u043fu0435u0447u0435u043du043eu0447u043du044bu043c powder contained methanol extractions of calculated salmon culture yell, shetland are colour different u0440u0430u0437u0432u0435u0434u0435u043du0438u00a0u043cu0438 diagnostic drug, mix thoroughly mix, closing the vial plug and u043eu0441u0442u0430u0432u043bu00a0u044eu0442 for one hour at room temperature, u0432u0441u0442u0440u00a0u0445u0438u0432u0430u00a0 every 5.10 min, then the mixture twice or three times for 10 min in u0446u0435u043du0442u0440u0438u0444u0443u0433u0438u0440u0443u044eu0442 1500...2000 about.mines, u043du0430u0434u043eu0441u0430u0434u043eu0447u043du0443u044e fluid after each u0446u0435u043du0442u0440u0438u0444u0443u0433u0438u0440u043eu0432u0430u043du0438u00a0 is blowing and u043fu0435u0440u0435u043du043eu0441u00a0u0442 into another test tube, after u0438u0441u0442u043eu0449u0435u043du0438u00a0 diagnostic drug u043fu0440u043eu0432u0435u0440u00a0u044eu0442 ph le u043eu0444u0438u043bu0438u0437u0438u0440u043eu0432u0430u043du043du043eu0433u043e diagnostic u0444u043bu044eu043eu0440u0435u0441u0446u0438u0440u0443u044eu0449u0435u0433u043e u0441u0430u043bu044cu043cu043eu043du0435u043bu043bu0435u0437u043du043eu0433u043e igg to antigens a, b, d, the scarred u0444u043bu044eu043eu0440u043eu0445u0440u043eu043cu043eu043c and u0434u043eu0432u043eu0434u00a0u0442 to u0437u043du0430u0447u0435u043du0438u00a0 7.2...7.4 with 0.15 normal saline NaOH, u0434u043bu00a0 u043fu0440u00a0u043cu043eu0439 reaction u0438u043cu043cu0443u043du043eu0444u043bu044eu043eu0440u0435u0441u0446u0435u043du0446u0438u0438 use 3.0...7.5 ml of diagnostic medicine, u0438u0441u0442u043eu0449u0435u043du043du043eu0433u043e immediately before it. u043fu0440u00a0u043cu043eu0439 reaction u0438u043cu043cu0443u043du043eu0444u043bu044eu043eu0440u0435u0441u0446u0435u043du0446u0438u0438, then u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 direct u043fu043eu0441u0442u0430u043d u043eu0432u043au0443 u043fu0440u00a0u043cu043eu0439 reaction u0438u043cu043cu0443u043du043eu0444u043bu044eu043eu0440u0435u0441u0446u0435u043du0446u0438u0438, u0434u043bu00a0 which slides prints with the organ or tissue at the incision u043du0430u043du043eu0441u00a0u0442 slides, laundered in u0445u0440u043eu043cu043fu0438u043au0435 and proto u0447u043du043eu0439 water and fat free in the mixture u043du0438u043au0438u0444u043eu0440u043eu0432u0430,drugs are dried at room temperature and subjected to a methanol for 15 min, the drugs u043du0430u043du043eu0441u00a0u0442 underweight u043bu0435u043eu0444u0438u043bu0438u0437u0438u0440u043eu0432u0430u043du043du044bu0439 u0434u0438u0430u0433u043du043eu0441u0442u0438u0447u0435u0441u043a three fluorescent u0441u0430u043bu044cu043cu043eu043du0435u043bu043bu0435u0437u043du044bu0439 immunoglobulin to antigens a, b, d, poorly u0444u043bu044eu043eu0440u043eu0445u0440u043eu043cu043eu043c, placed in moist chamber and u0438u043du043au0443u0431u0438u0440u0443u044eu0442 for 2 h at room temperature in u043fu0435u0440u0430u0442u0443u0440u0435, then launder four times for 5 min with 0.15 u043cu043eu043bu00a0u0440u043du044bu043c control valve.u043cu0438u043au0440u043eu0441u043au043eu043fu0438u0440u0443u044eu0442 with water u0438u043cu043cu0435u0440u0441u0438u0435u0439 or u0438u043cu043cu0435u0440u0441u0438u0435u0439 in u0444u043eu0441u0444u0430u0442u043du043eu043c buffer, drugs are microscopy on phosphorescent microscope with the following parameters:;lens water u0438u043cu043cu0435u0440u0441u0438u0438 u044560 / d = /.;u043eu043au0443u043bu00a0u0440 u044510,;exciting filter mop 15 - 2;locking filter u0436u0441 - 18 + u0436u0437u0441 - 19 - u0437u0435u043bu0435u043du0430u00a0 plate.