METHOD FOR OBTAINING SUBSTANCES OUT OF MAMMALIAN TISSUES AND ORGANS BEING OF AUTOIMMUNE ACTIVITY BOTH IN VITRO AND IN VIVO

摘要

FIELD: medicine, clinical immunology.;SUBSTANCE: material should be typified, homogenized with quartzitic sand and 0.9%-sodium chloride solution at 1:5 ratio, extracted by Grasse, at a 5-fold cycle frozen at -17 C and defrosted at +37 C, homogenate should be centrifuged to isolate supernatant and standardize there protein due to spectrometric technique 20-22 mg/ml and lyophilically dried, moreover, additionally, during the 4^th cycle of freezing it is necessary to conduct quantum treatment of a substance with gamma-quantums at 0.37 kJ energy at integral dose being 1.8 x 10⁴ Gr for 20-25 min. As a result, substances keep initial organic tissue specificity and are free of individual properties of isoantigenic form of donor's initial material. Combination of these features provides high accuracy of tissue identification in reactions in vitro and manifests its activity in vivo, as well. Substances have high purity of the substance as it is.;EFFECT: higher efficiency.;9 dwg, 1 ex