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CULTURE OF GENETICALLY NON-MODIFIED HUMAN CHONDROPROGENITORY CELLS AND METHOD FOR THEIR PREPARING

机译:非遗传修饰的人类软骨细胞的培养及其制备方法

摘要

FIELD: medicine, traumatology, orthopedics.;SUBSTANCE: invention relates to non-modified animal and human cells and methods for their preparing. The culture of genetically non-modified human chondroprogenitory cells obtained from human embryo hyaline cartilage at stage 1-2 trimesters of pregnancy comprises aggrecan- and collagen II-expressing cells in the amount 80%, not less. Suspension of primary dissociated human chondroprogenitory cells is obtained from human embryo cartilage tissue at stage 1-2 trimesters of pregnancy and cultured in selective cultural medium. Invention provides preparing homogenous cell culture for transplantation, enhanced effectiveness of treatment and avoiding undesirable results.;EFFECT: improved preparing method.;10 cl, 1 ex
机译:技术领域本发明涉及未经修饰的动物和人类细胞及其制备方法。在妊娠的1-2个月中从人胚胎透明软骨获得的基因未修饰的人软骨生殖细胞培养物中,含有表达聚集蛋白聚糖和胶原II的细胞的数量为80%,不少于80%。在怀孕的1-2个孕期从人胚胎软骨组织中获得原代解离的人软骨发育细胞的悬浮液,并在选择性培养基中进行培养。本发明提供了制备用于移植的均质细胞培养物,提高了治疗的有效性并避免了不良结果。效果:改进的制备方法; 10 cl,1 ex

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