PREPARATION, METHOD FOR ITS OBTAINING AND METHOD FOR QUICK TEST DIAGNOSTICS FOR ANTIGEN OF TICK-BORNE ENCEPHALITIS VIRUS

摘要

FIELD: medicine.;SUBSTANCE: the suggested preparation is being a 2%-coagglutinating reagent obtained due to adding equal amount of 0.1%-highly purified and high-avidity immunoglobulin against tick-borne encephalitis to 10%-staphylococcal reagent dissolved in distilled deionized water followed by sensitization for 1-1.5 h at 25-30 C, resuspending the residue in 0.15 M sodium chloride solution at pH 5.4-5.5 to obtain coagglutinating reagent up to 2%-content value. Method for quick test diagnostics deals with applying 15-20 mcl tested material onto a slide, adding equal amount of a certain preparation necessary for express diagnostics followed by thorough mixing by shaking a slide to visually determine the presence of antigen to tick-borne encephalitis virus in 2-7 min due to coagglutination phenomenon.;EFFECT: higher efficiency.;6 cl, 3 tbl