RECOMBINANT PROTEINS CONTAINING DIAGNOSIS SIGNIFICANT CORONAVIRUS PROTEIN ANTIGEN EPITOPE (SARS-COV), ASSOSIATED WITH GRIEVOUS ACUTE RESPIRATORY SYNDROME, AND SEQUENCES OF SYNTHETIC GENES ENCODING SARS-COV

摘要

FIELD: molecular biology, biotechnology, gene engineering, in particular diagnosis of atypical pneumonias.;SUBSTANCE: invention relates to DNA based on identified protein antigen epitope SARS-CoV. Particularly disclosed are nucleotide sequences of synthetic genes encoding recombinant protein fragments containing coronavirus protein antigen determinants SARS-CoV, represented in SEQ ID NO:1 - SEQ ID NO:8; as well as SARS-CoV virus protein fragments encoded by DNA sequences with amino acid sequences represented in SEQ ID NO:10 - SEQ ID NO:17. Said fragments are isolated by using preliminary obtained fusion protein by attachment to its N-terminal end GST-S-transferase protein with sequence represented in SEQ ID NO:9. Fusion proteins are useful in manufacturing of preparations for diagnosis of acute respiratory virus SARS-CoV.;EFFECT: new diagnostic agents for diagnosis of atypical pneumonias.;2 cl