METHOD FOR ISOLATION OF BIOACTIVE FRACTION (BAF) CONTAINING S-LIPOPOLYSACCHARIDES (S-LPS) FROM GRAM-NEGATIVE BACTERIA, BAF FOR PREVENTION AND TREATMENT OF DISEASES INDUCED BY ENDOTOXIC S-LPS PRODUCING GRAM-NEGATIVE BACTERIA, PHARMACEUTICAL COMPOSITION, METHODS FOR INDUCING OF PROTECTIVE IMMUNITY AND IMPROVING STATE IN PATIENT IN NEED OF IMMUNE STATUS INCREASING

摘要

FIELD: biotechnology, medicine.;SUBSTANCE: invention relates to method for isolation of bioactive fraction (BAF) containing essentially S-lipopolysaccharides (S-LPS) from endotoxic S-LPS producing gram-negative bacteria, useful in prophylaxis and therapy. From endotoxic S-LPS producing gram-negative bacteria BAF is isolated that has in lipid A S-LPS molar ratio of D-glucoseamine and -hydroxy acids selected from -hydroxydecanic, -hydroxydodecanic, -hydroxytetradecanic and -hydroxyhexadecanic acids of about 2:1-4, and molar ration of D-glucoseamine and high fatty acids connected via either amide or ester bond in lipid A S-LPS of about 2:3-7. Isolated BAF ins used in pharmaceutical composition to increase of patient immune status.;EFFECT: method for isolation from gram-negative bacteria bioactive fraction of S-LPS with high immunogenic properties, being capable to induce cytokine production with low pyrogenic and endotoxic properties.;41 cl, 4 dwg, 14 tbl, 24 ex