ISOLATED DNA MOLECULE ENCODING HUMAN ERYTHROPOIETIN (VARIANTS), EXPRESSING PLASMID OR VIRAL DNA-VECTOR, GLYCOPROTEIN ERYTHROPOIETIN (VARIANTS) AND METHOD FOR ITS PREPARING, PHARMACEUTICAL COMPOSITION (VARIANTS), MAMMALIAN CELL LINE (VARIANTS)

摘要

FIELD: biology, genetic engineering, biotechnology, medicine.;SUBSTANCE: invention relates to preparing glycosylated polypeptide (glycoprotein) as a component of human erythropoietin by using the technology of recombinant DNAs. This polypeptide shows ability to increase production of reticulocytes and erythrocytes, to enhance the level of hemoglobin synthesis and consumption of iron by marrow cells and characterized by the higher molecular mass as compared erythropoietin isolated from human urine. Invention describes variants DNA sequences encoding this polypeptide that comprise vector constructions with these sequences, a method for preparing transformed mammalian cell lines producing the recombinant human erythropoietin, and a method for its preparing and purification. Also, invention proposes pharmaceutical compositions comprising glycosylated polypeptide (glycoprotein) of erythropoietin as an active component. Applying this invention provides scaling the process for preparing active human erythropoietin useful for its using in medicine.;EFFECT: improved preparing method, valuable properties of polypeptide.;10 cl, 4 dwg, 21 tbl, 12 ex