A method for real time - quantifying a nucleic acid

摘要

The invention relates to a method for real time - quantifying a nucleic acid 10 by means of a nucleic acid - amplification reaction, wherein at least one with the nucleic acid 10 or their complementary counter strand 8 first forming a specific hybridization probe 18 or at least one intramolecular hybridization having a primer 12, 14 or at least one having an intramolecular hybridization, on the nucleic acid 10 or their complementary counter strand 8 specific binding second probe is present, the hybridization in the presence of the nucleic acid to be detected in the case of the nucleic acid - amplification reaction is removed or is formed, wherein said cancel each other or form of the hybridization electrochemically is detected, the first 18 or second probe or of the primer 12, 14 at least one electrochemically detectable label 20, is detected by means of which the electrochemical.