A method for the isolation of metal - cofactors of biologically - organic systems under application of the preparative native continuous polyacrylamide - gel electrophoresis (pnc - page)

摘要

The invention relates to a method for the isolation of metal - cofactors of biologically - organic systems under application of the preparative native continuous polyacrylamide - gel electrophoresis (pnc - page), characterized in that an acrylamide gel with a heating of 4% (w / v) is inserted, a electrophoresis buffer having a ph - value of 10,00 is used as well as 0,5 mul temed / ml of the gel and 5 mul aps / ml of gel are used, whereby the gel over a period of 69 hours is polymerized.