Fermentative preparation of L-lysine-containing product, involves inoculating and culturing bacterium in nutrient medium using coryneform bacteria through oxidative pentose phosphate pathway or tricarboxylic acid cycle

摘要

Fermentative preparation of an L-lysine-containing product using coryneform bacteria which produces L-lysine, involves inoculating and culturing the bacterium in a first nutrient medium using a coryneform bacteria having one or more carbon flows chosen from coryneform bacteria which direct the carbon flow through the oxidative pentose phosphate pathway, tricarboxylic acid cycle, citrate synthase reaction or aspartate kinase. Fermentative preparation (M1) of an L-lysine-containing product using coryneform bacteria which produces L-lysine, involves inoculating and culturing the bacterium in a first nutrient medium, where a concentration of the carbon source of not more than 10 g/l is established in the feed-in phase during the culturing, and using a coryneform bacteria having one or more, preferably two, or three or more carbon flows chosen from coryneform bacteria which direct the carbon flow through the oxidative pentose phosphate pathway with a percentage of more than 75%, coryneform bacteria which direct the carbon flow through the tricarboxylic acid cycle, referred to the acetyl radicals which are transferred by acetyl-CoA to oxaloacetate by the citrate synthase reaction, with a percentage of 1% but not more than 20%, coryneform bacteria which direct the carbon flow through aspartate kinase, coded for by lysC, with a percentage of 28% but not more than 60%, coryneform bacteria which direct the carbon flow through diaminopimelate dehydrogenase, coded for by ddh, with a percentage of 49% but not more than 98%, coryneform bacteria which direct the carbon flow through anaplerotic reactions, based on the sum of pyruvate and phosphoenol pyruvate (PEP), which are converted by PEP and pyruvate carboxylase, respectively, coded for by ppc and pyc, respectively, into oxaloacetate, with a percentage of more than 19%, coryneform bacteria which have the ability to establish a ratio of the carbon flow through the oxidative pentose phosphate pathway to the carbon flow through the anaplerotic reactions (oxidative pentose phosphate pathway [%]/anaplerotic reactions [%]=PPP/Ana[-]) of 3.4 but not more than 4.6, and coryneform bacteria which have the ability to establish a ratio of the carbon flow through the oxidative pentose phosphate pathway to the carbon flow into the tricarboxylic acid cycle (oxidative pentose phosphate pathway [%]/tricarboxylic acid cycle [%]=PPP/TCA[-]) of 7.