Preparing genetically modified Blakeslea, useful for preparation of carotenoids, useful as food additives, cosmetics or pharmaceuticals, comprises transformation, optional homokaryotizing, and selection

摘要

Preparing a genetically modified organism (A) of the genus Blakeslea comprises first transforming at least one cell; then optionally homokaryotizing the cells so that cells are produced in which the nuclei are all simultaneously altered in one or more genetic characteristics and these alterations are expressed; and finally selection and culture of the modified cell(s). Independent claims are also included for: (1) genetically altered, multinuclear cells of Blakeslea, especially B. trispora, produced by the new method; (2) two new promoters, of (2160 bp and 1520 bp, sequences fully defined in the specification) for use in the new method; (3) a terminator (774 bp, sequence fully defined in the specification), for use in the new method; and (4) a vector (15739 bp, sequence fully defined in the specification) for use in the new method.