Separation material for purifying His-tagged proteins, comprises carrier having a non-saturating concentration of active metal-chelating residues, allows elution at lower imidazole concentration

摘要

Separation material (A) for purification of His-tagged proteins (I) comprises a carrier to which metal ions are bound through an active chelating agent (X), the new feature being that (X) is present in a non-saturating concentration. An independent claim is also included for a column, for purifying (I), that contains (A).