A method for finding dephosphorylation phosphorylated by phosphatase activity of serine threonine or

摘要

The present invention relates to a process for detecting phosphatase activity in an immunoassay comprising the following steps:a) providing a protein or peptide comprising the sequence motif        -Z-X-Y-or        -Y-X-Z-whereinZ = serine or threonineX = a sequence of between 0 and 1000 amino acids which may be the same or differentY = tyrosine, serine or threonine as a substrate for a phosphatase, said protein or peptide being pre-phosphorylated at the Y and Z positions;b) incubating the protein or peptide with a phosphatase to form a protein or peptide which is dephosphorylated at the Z position;c) adding an antibody having a specificity to the peptide or protein that is phosphorylated in the Y and Z position; andd) detecting the phosphatase activity.