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In situ preparation of nucleoside - the group consisting of phosphoramidites and their use in the oligonucleotide synthesis
In situ preparation of nucleoside - the group consisting of phosphoramidites and their use in the oligonucleotide synthesis
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机译:原位制备核苷-亚磷酰胺组成的组及其在寡核苷酸合成中的用途
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摘要
The invention provides novel bifunctional phosphitylating reagents and their application in in situ preparation of 5'-protected nucleoside phosphoramidites and synthesis of oligonucleotides. Bifunctional phosphitylating reagents according to the invention react quickly with nucleosides under weakly acidic conditions. In addition, the bifunctional phosphitylating reagents according to the invention generate chemoselectively the corresponding nucleoside phosphoramidites in situ, without need to purify the nucleoside phosphoramidites before using them in oligonucleotide synthesis. Finally, the bifunctional phosphitylating reagents according to the invention are relatively stable and easy to handle.
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