A method for the analysis of the cell cycle of cell subpopulations from heterogeneous cell specimens

摘要

PROCEDURE FOR THE STUDY OF CELL CYCLE subpopulation CELLULAR SAMPLES cells in heterogeneous. INCLUDES: Incubate sample sequentially with: 1) A POOL MONOCLONAL ANTIBODY LABELED WITH ONE DIFFERENT fluorochrome 2) with a fluorochrome binds SPECIFIC TO NUCLEIC ACIDS; MEASURING Flow cytometric FLUORESCENT EMISSIONS; DETERMINING THE CELL CYCLE specific form of cell subpopulations SAMPLE IDENTIFIED BY ANALYSIS MULTIPARAMETRIC PERMITTED TO KNOW ALL TYPES CELLULAR IDENTIFIED THE PROPORTION OF CELLS IN PHASES G0 / G1, DNA synthesis (S) and G2 / mitosis ( G2 / M) CELL CYCLE.