Miniature device of the electrical detection of ligand type - receiver, 1994.

摘要

A miniature device (A) for electrical detection of ligand-receptor interactions comprising a solid support (S), electrically insulating, with a flat surface and carrying a matrix of nano-electrodes (NE), arranged in pairs, is new. A miniature device (A) for electrical detection of ligand-receptor interactions comprises a solid support (S), electrically insulating, with a flat surface and carrying a matrix of nano-electrodes (NE), arranged in pairs. The maximum distance between NE of a pair is 1 micron and the inter-electrode space (IES) is covered with an organic hydrophilic layer (A) for fixation of polypeptide probes (PP), covalently or by adsorption. (A) is in contact with NE and the device is connected to a system for measuring the electrical conductivity between each pair of NE. Independent claims are also included for: (1) a method for preparing (A); and (2) a method for electrical detection of ligand-receptor interactions using (A).