New evolved microorganisms with altered metabolic pathways, useful e.g. for production of amino acids, are selected as mutants able to grow on defined media

摘要

Method for preparing evolved microorganisms (A) with modified metabolic pathways. Method for preparing evolved microorganisms (A) with modified metabolic pathways comprises: (a) genetic modification of a microorganism to inhibit production or consumption of a metabolite when it is grown on a defined medium, thus affecting its ability to grow; (b) growing the modified organism in the defined medium so that evolution can occur, optionally with addition of a co-substrate to allow evolution; and (c) selecting as (A) cells able to grow on the medium, optionally in presence of co-substrate. Independent claims are also included for the following: (1) (A) produced by the new method; (2) method for producing an evolved protein (I) by culturing (A); (3) evolved gene (II) that encodes (I); and (4) (I) as new compounds.