New immunomodulator isolated from Bifidobacterium breve I-2219, useful as a pharmaceutical or food additive for regulating the intestinal microflora, especially suppression of Bacteriodes fragilis

摘要

An immunomodulator (A), isolated from Brevibacterium breveI-2219, is new. An immunomodulator (A) isolated from Brevibacterium breveI-2219 is new. It is prepared by: (a) incubation of I-2219, under aerobic or anaerobic conditions at 30-40 [deg]C, in an aqueous medium of pH 6-8 that contains whey permeate, whey protein hydrolyzate and lactose; (b) separation of I-2219 from the medium; (c) ultrafiltration of the medium on filter membranes with cut-off 100-300 kD to produce a concentrated retentate (CR); (d) dehydration of CR; (e) dissolving the product in buffer; (f) gel-exclusion chromatography on a column with cut-off 600 kD; and (g) recovering the excluded fraction as (A). ACTIVITY : Immunomodulatory; Antibacterial. Mice (18 animals; 10-12 weeks old) were treated with the excluded fraction from cultures of I-2219 as a replacement for their drinking water, and after 21 days their organs were examined for bacterial contamination. This was found in the kidneys in 7 animals; in the spleen of 3; in the liver of 5 and in the lungs of 2. For 23 animals given drinking water only, contamination was detected in kidneys in 12 animals; in the spleen in 13; in the liver of 10 and in the lungs of 11. MECHANISM OF ACTION : None given.