DOSAGE OF TECHIC ACIDS OF BACTERIA GRAM +

摘要

The subject of the invention is a method for the determination of the teichoic acids present most often in residual form, in a Gram + bacterial antigen preparation. This method firstly requires hydrolysis controlled by hydrofluoric acid at a temperature of less than or equal to 40 ° C in order to release the specific oligosaccharides of teichoic acids. The determination of the specific oligosaccharides can then be carried out by various techniques, in particular by high performance chromatography coupled with an amperometric detection in pulsed fields (HPAEC-PAD). The method according to the invention can be used in particular to assay the quantities. residuals of teichoic acids present in preparations containing capsular polysaccharides of Streptococcus pneumoniae, which may be useful as vaccines. / P