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Treating bone tissue, to form lipid- and virus-free implantable biomaterial, by treating successively with supercritical carbon dioxide, aqueous hydrogen peroxide, aqueous sodium hydroxide and ethanol
Treating bone tissue, to form lipid- and virus-free implantable biomaterial, by treating successively with supercritical carbon dioxide, aqueous hydrogen peroxide, aqueous sodium hydroxide and ethanol
Treatment of animal or human bone tissue, to form implantable biomaterial, involves: (a) mechanically cleaning to removal all surrounding organic material, while eliminating cartilage and osteophytes, (b) treating with a stream of supercritical carbon dioxide, (c) contacting with aqueous hydrogen peroxide solution under ultrasonication, (d) contacting with aqueous sodium hydroxide solution under ultrasonication, (e) rinsing with water and neutralizing, and (f) contacting with at least 90 vol. % ethanol solution then with at least 95 vol. % ethanol solution. An independent claim is included for implantable biomaterial consisting of bone tissue with a collagen framework, which is obtained by the above process, and has: (i) an overall lipid content of at most 0.4 (especially at most 0.1) wt. % and is free of all traces of chlorinated organic solvents, or (ii) has an overall lipid content of at most 0.08 wt. %.
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