首页> 外国专利> DNA SYNTHETIC ENZYME USING NONRADIOACTIVE LABEL AND METHOD FOR MEASURING ANTIBODY ACTIVITY TO THE SAME

DNA SYNTHETIC ENZYME USING NONRADIOACTIVE LABEL AND METHOD FOR MEASURING ANTIBODY ACTIVITY TO THE SAME

机译:非放射性标签的DNA合成酶及其抗体活性测定方法

摘要

PROBLEM TO BE SOLVED: To provide a method for measuring the activity of the DNA synthetic enzyme through one step in no need of the pretreatment such as concentration of the samples since it has the sufficiently high measurement sensitivity.;SOLUTION: In the reaction vessel in which a nucleotide chain as a template for the DNA synthetic enzyme is fixed, are introduced a reaction solution including a deoxyribonucleotide that is complementary to the nucleotide chain and is marked with the nonradioactive label, a primer and a surfactant and the specimen to carry out the DNA synthetic reaction. Then, the nonradioactive labels fixed to the vessel is measured.;COPYRIGHT: (C)2006,JPO&NCIPI
机译:解决的问题:提供一种无需进行诸如样品浓度之类的预处理就可以通过一个步骤测量DNA合成酶活性的方法,因为它具有足够高的测量灵敏度。将固定有作为DNA合成酶模板的核苷酸链的DNA引入反应溶液,该反应溶液包括与核苷酸链互补并用非放射性标记物标记的脱氧核糖核苷酸,引物和表面活性剂,并进行样品处理。 DNA合成反应。然后,测量固定在容器上的非放射性标记。COPYRIGHT:(C)2006,JPO&NCIPI

著录项

  • 公开/公告号JP2006187293A

    专利类型

  • 公开/公告日2006-07-20

    原文格式PDF

  • 申请/专利权人 OLYMPUS CORP;

    申请/专利号JP20060035310

  • 发明设计人 SUZUKI KAZUO;

    申请日2006-02-13

  • 分类号C12Q1/68;C12N15/09;G01N33/573;

  • 国家 JP

  • 入库时间 2022-08-21 21:56:35

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