MANUFACTURING METHOD FOR BUDDING YEAST CHD1 CHROMO-DOMAIN AND USE OF ITS NUCLEAR MAGNETIC RESONANCE SPECTRUM

摘要

PROBLEM TO BE SOLVED: To provide a manufacturing method for a budding yeast CHD1 chromo-domain and a nuclear magnetic resonance spectrum.;SOLUTION: This method is used for determining the existence, position, and/or intensity of interaction between the chromo-domain of budding yeast CHD1 and a test substance by utilizing the whole or part of the nuclear magnetic resonance spectrum of the budding yeast CHD1 chromo-domain. This manufacturing method is used for the chromo-domain of the budding yeast CHD1 or its variant including an amino-acid sequence described on any of sequence numbers 2, 4 or 6. This method includes cultivating a host transformed by DNA that encodes the chromo-domain of the budding yeast CHD1 or its variant including the amino-acid sequence described on any of the sequence numbers 2, 4 or 6, extracting the chromo-domain or its variant from culture, and gel-filtrating the extracted chromo-domain or its variant in concentrations of about 0.5 mM or less.;COPYRIGHT: (C)2006,JPO&NCIPI