METHOD FOR SCREENING ANTI-FLAVIVIRUS INFECTIOUS COMPOUND BY PERSISTENT FLAVIVIRUS-INFECTED CELL LINE

摘要

PPROBLEM TO BE SOLVED: To provide a method for screening a natural compound or an artificially synthesized compound against flavivirus by a persistent flavivirus-infected cell line. PSOLUTION: The screening method comprises the following steps: (a) preparing the persistent flavivirus-infected cell line; (b) producing a monoclonal antibody by using the persistent flavivirus-infected cell line; (c) bringing a previously selected compound into contact with the persistent flavivirus-infected cell line (cultivation with the compound); and (d) measuring the inhibiting activities of the selected compound against the flavivirus by an immunoenzyme method by using the monoclonal antibody. PCOPYRIGHT: (C)2006,JPO&NCIPI