Detection and identification of saxifylline using saxitoxin-biotin conjugates

摘要

A method for capturing a saxiphilin to allow for detection, characterisation, isolation an/or purification of said saxiphilin or its ligand, comprising: ( 1 ) providing a PST conjugate comprising a PST moiety bound via a linker through a site other than the binding site for saxiphilin directly or indirectly to a biotin moiety; ( 2 ) exposing the PST conjugate to a sample putatively containing said saxiphilin to create a reaction mixture and to (strept)avidin; and ( 3 ) allowing binding through the PST moiety to the saxiphilin and through the biotin moiety to (strept)avidin to form a captured PST complex.